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Amplified fragment length polymorphism‐derived microsatellite sequence linked to the Pch1 and Ep‐D1 loci in common wheat
Author(s) -
Groenewald J. Z.,
Marais A. S.,
Marais G. F.
Publication year - 2003
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.2003.00801.x
Subject(s) - biology , microsatellite , genetics , amplified fragment length polymorphism , allele , locus (genetics) , aegilops , common wheat , gene , cleaved amplified polymorphic sequence , microbiology and biotechnology , chromosome , restriction fragment length polymorphism , genetic diversity , ploidy , polymerase chain reaction , population , demography , sociology
Amplified fragment length polymorphism (AFLP) markers linked to the Aegilops ventricosa ‐derived chromosome segment in ‘VPM1’ on which the eyespot resistance gene, Pch1 , and the endopeptidase gene, Ep‐D1b , occur were identified. One marker was isolated from the gel, cloned and sequenced. Sequence analysis revealed a microsatellite repeat motif. Sequence‐specific primers were designed to amplify a product containing the repeat motif, and the microsatellite marker was tested for cosegregation with the Ep‐D1b allele. Distinct alleles were produced by the Pch1 sources, normal wheat and wheat containing the Lr19 translocation. A recombination frequency of 0.02 was calculated between the microsatellite marker and Ep‐D1.