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Morphological traits and high resolution RAPD markers for the identification of the main strawberry varieties cultivated in Argentina
Author(s) -
García M. G.,
Ontivero M.,
Diaz Ricci J. C.,
Castagnaro A.
Publication year - 2002
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.2002.00671.x
Subject(s) - rapd , biology , cultivar , dendrogram , genotype , genetic marker , botany , fragaria , horticulture , genetics , genetic diversity , gene , population , demography , sociology
Eight genotypes of the main Fragaria × ananassa cultivars grown in Argentina were analysed using the random amplified polymorphic DNA (RAPD) technique combined with electrophoresis in polyacrylamide gels. The high resolution of this procedure allowed the detection, with only 13 random primers, of 37 genotype‐specific bands that can be used as markers for verifying the identity of cultivars. By using this approach, three different accessions of the cultivar ‘Pájaro’ exhibited differences in amplification profiles, confirming the need for DNA analysis to prevent misidentification of cultivars. In addition, RAPD bands and morphological traits were used to assess genetic relatedness among cultivars. Comparison of both dendrograms revealed that there is no correlation between the clustering obtained with molecular and morphological characters.