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The use of AFLP markers for the identification of carrot breeding lines and F 1 hybrids
Author(s) -
GRZEBELUS D.,
SENALIK D.,
JAGOSZ B.,
SIMON P. W.,
MICHALIK B.
Publication year - 2001
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.2001.00647.x
Subject(s) - amplified fragment length polymorphism , biology , hybrid , dendrogram , genetics , primer (cosmetics) , genetic marker , amplicon , bulked segregant analysis , backcrossing , botany , genetic diversity , gene mapping , polymerase chain reaction , gene , population , chemistry , demography , organic chemistry , sociology , chromosome
Four inbred lines of carrot (cytoplasmic male‐steriles and corresponding maintainers) and eight of their F 1 hybrids were studied with the amplified fragment length polymorphism (AFLP) technique to examine their genetic relationship and produce markers useful for testing hybrid seed purity. Eighty‐six polymorphic amplicons were identified in bulked DNA samples using eight primer pair combinations. Genetic distance was estimated on the basis of the presence or absence of polymorphic bands. The dendrogram plotted on the basis of the AFLP data closely represented the pedigree relationships of the lines and their hybrids. From one to six amplicons specific for a breeding line were identified. Most of them were also present in the DNA bulks of respective F 1 hybrids. However, screening performed on individual plants of two parental lines and the corresponding hybrid indicated insufficient uniformity of parental lines, limiting the applicability of AFLP markers for testing hybrid seed purity.