Premium
Analysis of D‐prolamins synthesized by the Hordeum chilense genome and their effects on gluten strength in hexaploid tritordeum
Author(s) -
CABALLERO L.,
ALVAREZ J. B.,
MARTIN L. M.
Publication year - 2001
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.2001.00571.x
Subject(s) - biology , locus (genetics) , gluten , gel electrophoresis , storage protein , allele , polyacrylamide gel electrophoresis , genome , hordeum vulgare , cultivar , poaceae , botany , genetics , gene , biochemistry , enzyme
Hexaploid tritordeum is the amphiploid derived from the cross between Hordeum chilense and durum wheat. The storage proteins synthesized in the H ch genome influence the gluten strength of this amphiploid. The D‐prolamins of H. chilense have been analysed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis with and without urea. A new locus named Glu ‐ H ch 3 has been detected. The effects of allelic variation at this locus on gluten strength, as measured the sodium dodecyl sulphate sedimentation test, were determined using seeds of 92 lines from a cross of two hexaploid tritordeum lines. Two allelic variants have been detected for this locus, which have shown different effects on gluten strength.