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Molecular mapping of a new gene for resistance to frogeye leaf spot of soya bean in ‘Peking’
Author(s) -
Yang W.,
Weaver D. B.,
Nielsen B. L.,
Qiu J.,
Salamini F.
Publication year - 2001
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.2001.00563.x
Subject(s) - amplified fragment length polymorphism , biology , microsatellite , marker assisted selection , primer (cosmetics) , genetics , population , genetic marker , molecular marker , leaf spot , genetic linkage , cultivar , soya bean , gene , gene mapping , botany , allele , chromosome , genetic diversity , food science , chemistry , demography , organic chemistry , sociology
Amplified fragment length polymorphism (AFLP) and microsatellite (simple sequence repeat, SSR) techniques were used to map the _RGSp eking gene, which is resistant to most isolates of Cercospora sojina in the soya bean cultivar ‘Peking’. The mapping was conducted using a defined F 2 population derived from the cross of ‘Peking’(resistant) בLee’(susceptible). Of 64 Eco RI and MseI primer combinations, 30 produced polymorphisms between the two parents. The F 2 population, consisting of 116 individuals, was screened with the 30 AFLP primer pairs and three mapped SSR markers to detect markers possibly linked to Rcs Peking . One AFLP marker amplified by primer pair E‐AAC/M‐CTA and one SSR marker Satt244 were identified to be linked to Res Peking . The gene was located within a 2.1‐cM interval between markers AACCTA178 and Satt244, 1.1 cM from Satt244 and 1.0 cM from AACCTA178. Since the SSR markers Satt244 and Satt431 have been mapped to molecular linkage group (LG) J of soya bean, the Res Peking resistance gene was putatively located on the LG J. This will provide soya bean breeders an opportunity to use these markers for marker‐assisted selection for frogeye leaf spot resistance in soya bean.