Premium
Searching for molecular markers linked to male sterility and self‐compatibility in apricot
Author(s) -
Badenes M. L.,
Hurtado M. A.,
Sanz F.,
Archelos D. M.,
Burgos L.,
Egea J.,
Llácerl G.
Publication year - 2000
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.2000.00463.x
Subject(s) - biology , bulked segregant analysis , sterility , genetics , genetic marker , marker assisted selection , molecular marker , phenotype , allele , gene mapping , gene , chromosome
In apricot, there are two traits related to flower biology that can be a limitation for the breeder: self‐incompatibility and male sterility. Fully mature trees are needed to determine both phenotypes. Molecular biology techniques may provide a potential tool for increasing selection efficiency by means of marker‐assisted selection. In this study, randomly amplified polymorphic DNA markers combined with bulked segregant analysis have been used to search for markers linked to these traits. A screening of 228 primers resulted in a marker linked to male‐fertility (M4‐650). No markers linked to S alleles were obtained. A second approach consisted of the screening of primers in a subset of seedlings and resulted in three markers linked to male‐fertility and two markers linked to the Sc allele. These markers may allow an earlier determination of the phenotype in progenies segregating for these traits.