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Molecular markers linked to the Aegilops variabilis ‐derived root‐knot nematode resistance gene Rkn‐mn1 in wheat
Author(s) -
Barloy D.,
Lemoine J.,
Dredryver F.,
Jahier J.,
McIntosh R. A.
Publication year - 2000
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.2000.00451.x
Subject(s) - biology , rapd , locus (genetics) , root knot nematode , cultivar , genetic marker , genetics , gene , botany , chromosomal translocation , nematode , genetic diversity , population , ecology , demography , sociology
Aegilops variabilis no. 1 is the only known source of resistance to the root‐knot nematode Meloidogyne naasi in wheat. Previous studies showed that a dominant gene, Rkn‐mn1 , was transferred to a wheat translocation line from the donor Ae. variabilis. Random amplified polymorphic DNA (RAPD) analysis was performed on the wheat cultivar ‘Lutin’, on Ae. variabilis , on a resistant disomic addition line and on a resistant translocation line. For genetic and molecular studies, 114‐117 BC 3 F 2 plants and F 3 ‐derived families were tested. Five DNA and one isozyme marker were linked to Rkn‐mn1. Three RAPD markers flanking the Rkn‐mn1 locus were mapped at 0 cM (OpY16 ‐1065 ), 0.8 cM (OpB12 ‐1320 ) and 1.7 cM (OpN20 ‐1235 ), respectively. Since the Rkn‐mn1 gene remained effective, its introduction into different wheat cultivars by marker‐assisted selection is suggested.