Premium
Changes in expression of HMW glutenins of wheat ( Triticum aestivum L.) induced by nitrosoethylurea
Author(s) -
Š Vec M.,
Gregová E.,
Miklovičová M.,
Kraic J.
Publication year - 1999
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.1999.118003272.x
Subject(s) - glutenin , biology , mutant , gliadin , locus (genetics) , protein subunit , common wheat , storage protein , genotype , mutagenesis , gluten , genetics , botany , biochemistry , gene , chromosome
The application of a chemical mutagen, N‐nitroso‐N‐ethylurea, to the grains of wheat ( Triticum aestivum L.) cv. ‘Viginta’, provided a mutant line,‘NT‐10′, with an altered composition of high molecular weight (HMW) glutenin subunits. The qualitative mutation was detected in the Glu‐B1 locus by electrophoretic analyses of glutenins. Instead of the HMW glutenin subunits 7 + 9 present in the original genotype, a separate HMW subunit 6 was expressed in the mutant line. The other glutenin and gliadin proteins of the mutant line remained unchanged. The mutant line is also characterized by several changes in morphological and physiological characters—stronger stem, wider leaf, bigger spike and higher grain hardness. This is the first communication of the possibility of changing the composition of high molecular weight subunits of wheat glutenin by means of mutagenesis.