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Male sterility in alloplasmic Brassica rapa L. carrying Eruca sativa cytoplasm
Author(s) -
Matsuzawa Y.,
Mekiya S.,
Kaneko Y.,
Bang S. W.,
Wakui K.,
Takahata Y.
Publication year - 1999
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.1999.118001082.x
Subject(s) - biology , cytoplasmic male sterility , brassica rapa , sterility , backcrossing , eruca , stamen , botany , vernalization , brassica , pollen , gene , genetics , photoperiodism
For the development of a new cytoplasmic male sterility (CMS) system in Brassica rapa (2 n = 20, AA), intergeneric hybridization was performed in Eruca sativa (2 n = 22, EE)× B. rapa. The original amphihaploid F 1 plant (2 n = 21, EA) generated via embryo rescue produced a sesquidiploid F 1 plant (2 n = 31, EAA), from which the alloplasmic F 3 plants were generated. In F 3 , some progenies with malformed anthers were maintained as male‐sterile lines up to the F5 generation. In the F 6 and F 7 generations, the alloplasmic male‐sterile plants were bred by backcrossing to several B. rapa genotypes and were then classified into the three distinctive types: petaloid, antherless and brown anther, in addition to three intermediate types between them. By southern blot analysis, each plant of the petaloid and antherless types was shown to carry the cytoplasm genome of E. sativa. These male‐sterile plants produced as many viable seeds as the corresponding male‐fertile plants, although their nectary gland development was minimal. Consequently, CMS lines of the petaloid and antherless types with enhanced seed fertility and nectary gland development could provide promising plant materials for F 1 seed production in B. rapa.