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Gliadins as biochemical markers for Aegilops turcomanica genes in wheat lines
Author(s) -
Vahl U.,
Bringezu T.,
Muller G.
Publication year - 1999
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1046/j.1439-0523.1999.00392.x
Subject(s) - biology , introgression , gliadin , powdery mildew , aegilops , doubled haploidy , common wheat , glutenin , ploidy , polyacrylamide gel electrophoresis , storage protein , cultivar , poaceae , gel electrophoresis , gene , botany , genetics , gluten , biochemistry , chromosome , protein subunit , enzyme
An Aegilops turcomanica ‐typical gliadin was discovered in sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) patterns of ethanol‐soluble seed proteins from wheat lines Tr. 476, 482 and 492, which had been derived from a direct cross of Ae. turcomanica and Triticum aestivum and which revealed powdery mildew resistance due to a putative Ae. turcomanica ‐introgression. This Ae. turcomanica ‐derived gliadin was tested for its suitability as biochemical marker. For this purpose, doubled‐haploid lines were produced via anther culture from crosses of Tr. 482 and Tr. 492 with actual winter wheat cultivars and breeding lines. Until now, 173 lines with Ae. turcomanica ‐gene(s) have been selected from 297 doubled haploid wheat lines.