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Antifungal susceptibility of clinical and environmental isolates of Cryptococcus neoformans to four antifungal drugs determined by two techniques
Author(s) -
Moraes E. M. P.,
Prímola N. S.,
Hamdan Júnia Soares
Publication year - 2003
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1046/j.1439-0507.2003.00872.x
Subject(s) - amphotericin b , cryptococcus neoformans , fluconazole , itraconazole , flucytosine , antifungal , microbiology and biotechnology , cryptococcosis , cryptococcus , cryptococcus gattii , antifungal drugs , biology , antifungal drug , minimum inhibitory concentration , fungus , drug resistance , antibiotics , botany
Summary A total of 64 Cryptococcus neoformans strains, including clinical and environmental Brazilian isolates var. neoformans and var. gattii , were tested for susceptibility to amphotericin B, 5‐flucytosine, fluconazole and itraconazole. The tests were performed according to the recommendations of National Committee of Clinical Laboratory Standards and the method of macrodilution in liquid medium of Shadomy et al. [ Manual de Microbiologia Clínica , 4th edn. Buenos Aires: Editorial Medica Panamericana, 1987: 1229–38]. For most drugs there was a significant difference between the readings taken at 24 and 48 h with both methods. When the minimum inhibitory concentrations obtained by the two techniques were compared, significant differences were observed for amphotericin B and fluconazole. Overall, differences in drug susceptibility with respect to the origin of the isolates or the variety of the fungus were not observed. As an exception, the gattii variety exhibited a high resistance rate to amphotericin B when the technique of Shadomy et al . was applied, a fact possibly related to the greater difficulty for treatment of the disease caused by this fungal variety.