Premium
Enzymuria following amphotericin B application in the rat
Author(s) -
Inselmann Gerhard,
Balaschke Michael,
Heidemann Hugo T.
Publication year - 2003
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1046/j.1439-0507.2003.00856.x
Subject(s) - creatinine , amphotericin b , excretion , renal function , medicine , endocrinology , chemistry , kidney , urinary system , antifungal , dermatology
Summary The effect of amphotericin B application on urinary renal tubule enzyme excretion was investigated in rats treated with amphotericin B (1.5 mg kg −1 b.i.d., i.v.) for 4 days. Application of amphotericin B induced a significant higher daily urinary enzyme activity of the renal tubular enzymes N ‐acetyl‐β‐glucosaminidase (NAG), β‐glucuronidase (GRS), alanine‐aminopeptidase (AAP) and γ‐glutamyltransferase (GGT) in comparison with controls and sodium deoxycholate treated animals as well. A significant increase in the renal excretion of NAG, GRS, AAP and GGT occurred after the first day of amphotericin B treatment and continued until the fourth day. Following treatment for 4 days with amphotericin B urine AAP activity amounted to 69 ± 19 U g −1 creatinine, control: 39 ± 7 U g −1 creatinine ( P < 0.05). After 4 days GGT excretion increased to 803 ± 238 U g −1 creatinine, control: 445 ± 106 U g −1 creatinine ( P < 0.05). At the fourth day NAG excretion was 80 ± 39 U g −1 creatinine, control: 23 ± 5 U g −1 creatinine ( P < 0.05) and GRS 724 ± 604 U g −1 creatinine (amphotericin B), control: 276 ± 158 U g −1 creatinine ( P < 0.05). Treatment with amphotericin B decreased the creatinine clearance significantly: 0.94 ± 0.16 ml −1 min −1 vs. control 1.35 ± 0.29 ml −1 min −1 ( P < 0.05). Fractional sodium and potassium excretion was not influenced by amphotericin B. The application of sodium deoxycholate had no influence on urinary renal tubular enzyme activity. The results show that amphotericin B application induces early enzymuria of renal tubule enzymes suggesting damage of proximal renal tubules.