Premium
Fallbericht. Molekularbiologische Identifizierung eines Lungen‐Aspergilloms und molekularbiologische Isolat‐Typisierung aus Sputum und Autopsiematerial
Author(s) -
Sugita C.,
Makimura K.,
Murakami A.,
Murai Y.,
Yamaguchi H.,
Nagai A.
Publication year - 2003
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1046/j.1439-0507.2003.00850.x
Subject(s) - aspergilloma , sputum , aspergillus fumigatus , fungus , microbiology and biotechnology , aspergillus , biology , typing , pulmonary aspergillosis , aspergillosis , autopsy , ribosomal rna , tuberculosis , medicine , pathology , immunology , genetics , botany , gene
Summary Filamentous fungi were isolated from antemortem sputum and an autopsy fungus ball of the lung in a case of aspergilloma. Both of the isolates were analyzed for the sequences of species or strain‐specific nuclear ribosomal DNA (partial 28S and ITS1 regions), and were identifed as Aspergillus fumigatus . The molecular biological technique saved time and is thought to be a powerful tool in the accurate diagnosis of pulmonary fungal infection to assure effective treatment.