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Histopathologic and histochemical characterization of calcified structures in hamsters inoculated with Paracoccidioides brasiliensis
Author(s) -
Essayag S. M.,
Landaeta M. E.,
Hartung C.,
Magaldi S.,
Spencer L.,
Suárez R.,
García F.,
Pérez E.
Publication year - 2002
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1046/j.1439-0507.2002.00785.x
Subject(s) - von kossa stain , paracoccidioides brasiliensis , haematoxylin , pathology , staining , eosin , inoculation , biology , calcification , nocardia brasiliensis , h&e stain , anatomy , microbiology and biotechnology , medicine , bacteria , biochemistry , enzyme , alkaline phosphatase , genetics , nocardia , nocardiosis
Summary. In pathogenicity studies of 31 Paracoccidioides brasiliensis isolates preserved using Castellani's method we intraperitoneally inoculated 104 young adult hamsters and found laminated concentric structures and calcified appearance that resembled Schaumann bodies, in 43 of them, especially in animals with apparently good condition. We characterized these structures histologically and histochemically using different stains (PAS, Grocott, haematoxylin‐eosin, Von Kossa). The Von Kossa staining revealed calcium in these structures. Similar structures have been described in patients with sarcoidosis and also in hamsters inoculated with P. brasiliensis. We found no correlation between the presence of these calcifications and serum calcium levels.