Premium
Empfindlichkeitsprüfung an Malassezia pachydermatis mittels Harnstoff‐Mikrodilutionsmethode
Author(s) -
Murai T.,
Nakamura Y.,
Kano R.,
Watanabe S.,
Hasegawa A.
Publication year - 2002
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1046/j.1439-0507.2002.00733.x
Subject(s) - bifonazole , broth microdilution , lysozyme , itraconazole , terbinafine , microbiology and biotechnology , minimum inhibitory concentration , biology , econazole , chemistry , miconazole , antimicrobial , antifungal , biochemistry
Summary. The in vitro susceptibility of 24 isolates of Malassezia pachydermatis to four antifungal drugs in combination with lysozyme was determined using a urea broth microdilution method. The antifungal activities of each drug alone against 24 isolates of M. pachydermatis were determined as the mean minimal inhibitory concentrations (MICs). MICs of bifonazole, itraconazole, amorolfine and terbinafine were 3.2 µg ml −1 , 1.6 µg ml −1 , 25 µg ml −1 and 3.2 µg ml −1 , respectively. Lysozyme alone inhibited the growth of M. pachydermatis in a dose‐dependent manner, although the lysozyme was unable to kill the cells of M. pachydermatis at the highest concentration of 20 µg ml −1 . Furthermore, the mean MICs of bifonazole, itraconazole, amorolfine and terbinafine in combination with lysozyme were the same as the results for each drug alone. Although the activity of antifungal drugs in combination with lysozyme is enhanced for other fungi. These results suggested that M. pachydermatis might not be affected by the host's natural defences.