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Colorimetric method for susceptibility testing of voriconazole and other triazoles against Candida species
Author(s) -
Kauffman Carol A.,
Zarins Lidija T.
Publication year - 1999
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1046/j.1439-0507.1999.00511.x
Subject(s) - voriconazole , itraconazole , fluconazole , candida glabrata , serial dilution , candida albicans , microbiology and biotechnology , minimum inhibitory concentration , candida krusei , broth microdilution , biology , antifungal , medicine , antimicrobial , pathology , alternative medicine
A microdilution assay using Alamar Blue, a colorimetric indicator, was compared with the NCCLS macrodilution broth assay for voriconazole, fluconazole, and itraconazole against Candida albicans , Candida glabrata , and Candida krusei . Concordance (±2 dilutions) between the two methods was highest for voriconazole (98.3%), and for fluconazole and itraconazole it was 94.3 and 95.4%, respectively. Twenty‐six of 32 (81.2%) discordant readings (≥3 dilutions different) were noted in C. glabrata isolates, and all but two isolates showing discordance had higher minimum inhibitory concentration readings with the colorimetric method.