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Detection of sugars and aminoacids in antigens of Trichophyton mentagrophytes var. erinacei
Author(s) -
Adnan Hamad Aubaid,
Afrah Z. Risan,
A K Naem
Publication year - 1999
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1046/j.1439-0507.1999.00459.x
Subject(s) - sephadex , antigenicity , glycopeptide , fractionation , chromatography , chemistry , sugar , phenol , mycelium , trichophyton , amino acid , antigen , biochemistry , ethylene glycol , biology , microbiology and biotechnology , organic chemistry , botany , immunology , antibiotics , antifungal , enzyme
Crude antigen extracted from mycelium of Trichophyton mentagrophytes var. erinacei by means of phenol and ethylene glycol was studied for sugar and amino acid constituents using DEAE‐Sephadex A‐50 column chromatography for fractionation. The results revealed that the crude antigen contains 13 sugars and 15 amino acids. It is presumed that the antigenicity is based on glycopeptide fractions.