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Occurrence of Antimicrobial Serin‐Proteinases in Sunflower Seeds
Author(s) -
Mendieta J. R.,
Giudici A. M.,
Canal L.
Publication year - 2004
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.2003.00799.x
Subject(s) - sclerotinia sclerotiorum , biology , sunflower , trypsin , antimicrobial , trypsin inhibitor , germination , spore germination , microbiology and biotechnology , antifungal , sclerotinia , pathogen , biochemistry , botany , enzyme , horticulture
Using an experimental approach directed to the isolation of antimicrobial proteins, we have detected the presence of a trypsin inhibitor (TI) with associated antifungal activity in sunflower seeds. Purification of the isolated protein by affinity chromatography on a trypsin‐agarose matrix confirmed that a trypsin inhibitor was responsible for the inhibition of spore germination of the fungal pathogen Sclerotinia sclerotiorum . The protein is a potent antifungal compound as it can completely inhibit the germination of S. sclerotiorum ascospores at a concentration of 14 μ g/ml. The putative contribution of this TI to control fungal invasion is discussed.