Premium
Improvement of RT‐PCR Based Detection of Two Closteroviruses Associated with Little Cherry Disease in Sweet Cherries
Author(s) -
Rybak M.,
Kountrias A.,
Eppler A.,
Jelkmann W.,
Heinze C.,
Adam G.
Publication year - 2004
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.2003.00793.x
Subject(s) - biology , bark (sound) , primer (cosmetics) , horticulture , sampling (signal processing) , fruit tree , real time polymerase chain reaction , botany , virology , gene , genetics , ecology , computer science , computer vision , chemistry , organic chemistry , filter (signal processing)
The increasing availability of nucleotide sequence data from plant pathogenic fruit tree viruses led recently to the development of nucleic‐acid‐based detection methods as a routine tool for diagnosis. We present a simplification and modification of the previously described test procedure for the two closteroviruses Little cherry virus 1 and 2 (LChV‐1, LChV‐2). For LChV‐1, a single tube reaction reduced unspecific amplification that led to false positive results. For the detection of LChV‐2, a primer set was developed for the local isolates of the area ‘Altes Land’ in the northern part of Germany. To determine the optimal sampling conditions for a reliable detection of both viruses different types of tissue (bark, bud, leaves) were tested in monthly intervals.