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Effect of Metabolic Inhibitors on Red Light‐induced Resistance of Broad Bean ( Vicia faba L.) against Botrytis cinerea
Author(s) -
Rahman M. Z.,
Honda Y.,
Islam S. Z.,
Arase S.
Publication year - 2002
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.2002.00781.x
Subject(s) - cycloheximide , botrytis cinerea , biology , dcmu , spore , spore germination , botany , germination , photosynthesis , inoculation , horticulture , protein biosynthesis , biochemistry , photosystem ii
A possible relationship between the synthetic activities of broad bean leaves and red light‐induced resistance against Botrytis cinerea was examined. Both lesion formation and fungal development were suppressed under red light irradiation. In red light‐irradiated leaflets, restricted chocolate lesions were observed only on their inoculated adaxial surfaces, whereas black lesions extended to non‐inoculated abaxial surfaces in the dark. In microscopic studies, clear cell browning was recognized only in red light‐irradiated leaves. Treatment of leaves with an inhibitor of photosynthesis, 3‐(3,4‐dichlorophenyl)‐1,1‐dimethyl urea (DCMU) (25 μ m ), and an inhibitor of protein synthesis, cycloheximide (25 μ g/ml), inhibited the induction of resistance by red light irradiation. Moreover, DCMU‐ and cycloheximide‐treated leaflets produced black necrotic lesions even under red light irradiation for 48 h, which extended to the non‐inoculated abaxial surface. DCMU did not affect fungal growth, spore production or spore germination of B. cinerea . By contrast, cycloheximide at 25 μ g/ml suppressed mycelial growth, but not spore production, whereas cycloheximide at the same concentration completely inhibited spore germination. These results suggest that red light‐induced resistance possibly depends on host photosynthesis and protein synthesis.