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Isolation of an RNA‐dependent RNA Polymerase from Nicotiana benthamiana Plants Infected with Tobacco Necrosis Virus (strain D) and Template‐dependent Transcription of its Satellite, Satellite Tobacco Necrosis Virus (strain C) RNA
Author(s) -
Coutts,
Plante
Publication year - 2001
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.2001.00603.x
Subject(s) - nicotiana benthamiana , biology , rna dependent rna polymerase , rna polymerase , rna , tobacco mosaic virus , virology , nicotiana tabacum , polymerase , satellite , transcription (linguistics) , tobacco etch virus , virus , plant virus , gene , biochemistry , potyvirus , linguistics , philosophy , engineering , aerospace engineering
Partially purified RNA‐dependent RNA polymerase activity isolated from Nicotiana benthamiana plants infected with Tobacco necrosis virus strain D (TNV‐D) is capable of template‐dependent synthesis of Satellite tobacco necrosis virus strain C (STNV‐C) RNA. This in vitro system will be of great value in investigations on both TNV‐D and STNV‐C RNA replication.