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Detection of Phytoplasma Infection in Rose, with Degeneration Symptoms
Author(s) -
Kamińska M.,
Dziekanowska D.,
RudzińskaLangwald A.
Publication year - 2001
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.2001.00554.x
Subject(s) - phytoplasma , biology , aster yellows , catharanthus roseus , restriction fragment length polymorphism , polymerase chain reaction , phloem , sieve tube element , restriction enzyme , botany , nested polymerase chain reaction , horticulture , dna , genetics , gene
In 1998 a severe disease was observed on rose cvs. ‘Patina’, ‘Papillon’ and ‘Mercedes’ cultivated in a commercial greenhouse in Poland. The symptoms included stunted growth, bud proliferation, leaf malformation and deficiency of flower buds. Sporadically some plants yielded flower buds transformed into big‐bud structures and degenerated flowers. The presence of phytoplasma in roses with severe symptoms as well as in recovered plants and Catharanthus roseus experimentally infected by grafting and via dodder was demonstrated by nested polymerase chain reaction assay with primers pair R16F2/R2 or R16F1/R0 and R16(I)F1/R1 amplifying phytoplasma 16S rDNA fragment. The polymerase chain reaction products (1.1 kb) used for restriction fragment length polymorphism analysis after digestion with endonuclease enzymes Alu I and Mse I produced the same restriction profiles for all samples. The restriction profiles of phytoplasma DNA from these plants corresponded to those of an aster yellows phytoplasma reference strain. Electron microscope examination of the ultra‐thin sections of the stem showed wall thickenings of many sieve tubes of the diseased roses and single phytoplasma cells within a sieve element of the phloem of experimentally infected periwinkles. This paper is the first report on aster yellows phytoplasma in rose identified at a molecular level.