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Wirksamkeit vier neuer Pyrazol‐Pyrimidine gegen Phytopathogene: Feinstrukturelle Nachweise bei Pythium ultimum
Author(s) -
Mares D.,
Romagnoli C.,
Sacchetti G.,
Fabiano A.,
Vicentini C. B.,
Bruni A.
Publication year - 2000
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.2000.00526.x
Subject(s) - pythium ultimum , biology , ultrastructure , botrytis cinerea , vesicle , vacuole , hypha , botrytis , phosphatidylcholine , biochemistry , microbiology and biotechnology , botany , cytoplasm , membrane , rhizoctonia solani , phospholipid
Four newly synthesized molecules derived from pyrazole‐pyrimidine were assayed on Botrytis cinerea Micheli, Fusarium moniliforme Sheld and Pythium ultimum Trow. All proved effective in inhibiting the growth of the phytopathogens at all of the test concentrations (10, 20, 50, 100 μg/ml). The most effective compound was 1‐(3)nitrophenyl ‐ 6 ‐ trifluoromethylpyrazolo[3,4 ‐ d ]pyrimidine 4(5H)‐thione (CF 3 3). Ultrastructural studies on P. ultimum treated with CF 3 3 revealed alterations in the normal hyphal shape and, at high concentration, plasmolysis and damage to the wall texture was observed. At 20 μg/ml different vesicles were seen in the cytoplasm: some appeared quite dense, and specific cytochemical reactions indicated that they were most likely peroxysomes; other vesicles seem to be vacuoles of varying content. In some cases there was disintegration of the nuclear envelope. The effects on membrane lipids and interference in protein synthesis are hypothesized as possible mechanism of action of the molecule.