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A Novel Satellite RNA of Cucumber Mosaic Virus Induces Unique Line‐pattern Mosaic Symptoms in Tobacco
Author(s) -
Sato H.,
Hase S.,
Sugiyama M.,
Karasawa A.,
Suzuki T.,
Takahashi H.,
Ehara Y.
Publication year - 2000
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.2000.00469.x
Subject(s) - biology , tobacco mosaic virus , mosaic , cucumber mosaic virus , satellite , virology , rna , line (geometry) , virus , plant virus , genetics , gene , geometry , mathematics , archaeology , engineering , history , aerospace engineering
The CMV(YW) isolate of cucumber mosaic virus (CMV) induced unique line‐pattern mosaic symptoms in systemically infected leaves of tobacco ( Nicotiana tabacum cv. Ky57). By northern hybridization analysis using cDNA to CMV(Y) satellite RNA as a probe, it was confirmed that CMV(YW) contained a satellite RNA. which was designated sat‐YW RNA; this was 388 nucleotides in length and did not have either a conserved domain that induces necrosis in tomato or chlorosis in tobacco. CMV(YW) free of sat‐YW RNA. which was isolated by the single lesion isolation method using Chenopodium amaranticolor , did not induce the unique line‐pattern mosaic symptom. Furthermore, the sat‐YW RNA‐mediated line‐pattern mosaic symptom was also induced by in vitro transcribed infectious sat‐YW RNA in tobaccos infected with either CMV(YW) or CMV(Y) genomic RNA. These results clearly demonstrated that sat‐YW RNA induces the unique line‐pattern mosaic symptom on CMV‐infected tobaccos.