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Charakterisierung von Trichoderma ‐Isolaten aus philippinischen Reisfeldern durch UP‐PCR und rDNA‐ITS1‐Analyse: Identifizierung von UP‐PCR‐Markern
Author(s) -
Cumagun C. J. R.,
Hockenhull J.,
Lübeck M.
Publication year - 2000
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.2000.00467.x
Subject(s) - biology , internal transcribed spacer , ribosomal dna , polymerase chain reaction , trichoderma harzianum , trichoderma , primer (cosmetics) , ribosomal rna , restriction fragment length polymorphism , spacer dna , restriction enzyme , 16s ribosomal rna , microbiology and biotechnology , genetics , botany , phylogenetic tree , dna , gene , biological pest control , chemistry , organic chemistry
Forty‐two isolates of Trichoderma from rice fields in four provinces in the Philippines were characterized using rDNA‐ITS1 analysis and universally primed polymerase chain reaction (UP‐PCR). Two groups were clearly distinguishable on the basis of length and restriction pattern of the internal transcribed spacer (ITS) region of the ribosomal DNA and UP‐PCR banding profiles using UP primer, L45. The 40 isolates comprising the largest group were very similar with respect to their UP‐PCR banding profiles and were assigned to Trichoderma harzianum Rifai following morphological identification of four of the isolates. The two isolates belonging to the second group were identified as Trichodermaviride Pers. ex. Gray on the basis of their morphology, rDNA‐ITS1 analysis and distinct UP‐PCR banding profiles. One of the T. harzianum isolates with good cellulolytic and competitive saprophytic abilities was analysed using single and pair‐wise combinations of UP primers in order to distinguish it from the remaining 41 isolates. A suitable diagnostic marker was identified and this marker will be valuable for monitoring the isolate in field tests.