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Identification of Toxigenic Fusarium Species using PCR Assays
Author(s) -
Chelkowski J.,
Bateman G. L.,
Mirocha CH. J.
Publication year - 1999
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.1999.147005307.x
Subject(s) - biology , fusarium , fusarium culmorum , rapd , polymerase chain reaction , primer (cosmetics) , mycotoxin , fungi imperfecti , botany , microbiology and biotechnology , genetics , genetic diversity , gene , population , chemistry , demography , organic chemistry , sociology
Isolates of the toxigenic cereal pathogens Fusarium culmorum , Fusarium graminearum , Fusarium crookwellense and Fusarium avenaceum , from Poland (48 isolates) and 12 from England, New Zealand, Italy and Canada, were examined using random amplified polymorphic DNA (RAPD)‐polymerase chain reaction (PCR), sequence‐characterized amplified regions (SCARs), morphology and mycotoxin production under laboratory conditions. Their DNA products were compared by RAPD‐PCR, which showed species‐specific bands and the greatest diversity among isolates of F. avenaceum. PCR using three 20‐mer‐primer‐pairs that are reported to be useful for identification of F. culmorum and F. graminearum group 2 confirmed their species‐specificity. The same species‐specific PCR product was observed in isolates of both nivalenol and deoxynivalenol chemotypes of F. culmorum or F. graminearum. A clear relationship was found between morphological and species‐specific PCR identification of F. culmorum and F. graminearum isolates. However, F. avenaceum can be confused when using primers FA‐ITS F/R (SCAR 2‐14) with Fusarium tricinctum because the same band 272 bp appears in the gel, in both species probes.