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Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Author(s) -
Rosner A.,
Maslenin L.
Publication year - 1999
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.1999.00055.x
Subject(s) - biology , potato virus y , virology , restriction enzyme , polymerase chain reaction , strain (injury) , virus , genetics , rna , virus strain , microbiology and biotechnology , dna , gene , plant virus , anatomy
PVY NTN was distinguished from PVY N on the basis of differences in electrophoretic mobility of their corresponding RNA transcripts. Transcripts were copied from polymerase chain reaction products of two PVY strains to which the T7 promoter was added. Single‐strand and double‐strand transcript heteroduplexes of the NTN and N strains exhibited different rate of migration in gel which enabled strain differentiation. This procedure obviates the need for prior information on sequence divergence (e.g. restriction sites, etc.) among the virus isolates.