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Identification of microsatellite DNA markers for population structure analysis in Indian major carp, Cirrhinus mrigala (Hamilton‐Buchanan, 1882)
Author(s) -
Lal K. K.,
Chauhan T.,
Mandal A.,
Singh R. K.,
Khulbe L.,
Ponniah A. G.,
Mohindra V.
Publication year - 2004
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1046/j.1439-0426.2003.00538.x
Subject(s) - biology , microsatellite , loss of heterozygosity , intraspecific competition , carp , genetics , population , zoology , allele , genetic divergence , genetic diversity , fish <actinopterygii> , fishery , gene , demography , sociology
Summary Forty‐four primer sequences available for four cyprinid fishes were tested to amplify microsatellite loci in Indian major carp, Cirrhinus mrigala . A total of 12 loci were successfully amplified with clear scorable patterns and five thereof were polymorphic. Suitability of the identified polymorphic loci in population structure analysis of C. mrigala was assessed. Genetic variation was examined in 76 specimens collected from five different rivers. The mean observed heterozygosity ranged from 0.247 to 0.333. Significant heterogeneity in allele frequencies was detected, indicating that the samples analysed did not belong to homogenous populations. The identified microsatellite markers are promising for the analysis of intraspecific divergence in C. mrigala across its distribution range.