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Vaccination of African catfish with Vibrio anguillarum O2 : II. Oral intubation with vaccine‐containing pellets
Author(s) -
Vervarcke S.,
Lescroart O.,
Ollevier F.,
Kinget R.,
Michoel A.
Publication year - 2004
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1046/j.1439-0426.2003.00536.x
Subject(s) - vibrio anguillarum , catfish , biology , microbiology and biotechnology , antigen , vaccination , immune system , antibody , immunology , vibrio , bacteria , fish <actinopterygii> , genetics , fishery
Summary The aim of this study was to investigate the success of oral vaccination application in African catfish using Vibrio anguillarum O2 bacterins. The antigen uptake was followed by competitive enzyme‐linked immunosorbent assay (ELISA). Serum antibody response was measured using an indirect ELISA. Several in vivo administration methods were investigated. Intraperitoneal injection gave the highest absorption rate, with high antibody levels in the systemic circulation. Oral intubation of bacterin‐layered pellets resulted in low antigen uptake and low antibody levels. The addition of absorption enhancers increased the serum antigen levels. An enteric coating applied on the pellets containing vaccine did not improve the immune response.