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Characterization of novel Brazilian Bacillus thuringiensis strains active against Spodoptera frugiperda and other insect pests
Author(s) -
Silva S. M. B.,
SilvaWerneck J. O.,
Falcão R.,
Gomes A. C.,
Fragoso R. R.,
Quezado M. T.,
Neto O. B. O.,
Aguiar J. B.,
Sá M. F. G.,
Bravo A.,
Monnerat R. G.
Publication year - 2004
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1046/j.1439-0418.2003.00812.x
Subject(s) - bacillus thuringiensis , biology , anticarsia gemmatalis , spodoptera , cry1ac , culex quinquefasciatus , lepidoptera genitalia , aedes aegypti , microbiology and biotechnology , sf9 , strain (injury) , botany , noctuidae , larva , gene , biochemistry , recombinant dna , bacteria , genetics , genetically modified crops , transgene , anatomy
Brazilian strains of Bacillus thuringiensis , namely S701, S764 and S1265 were analysed regarding their cry gene and protein contents, crystal type, and activity against larvae of the lepidopteran fall armyworm ( Spodoptera frugiperda Smith), the velvet caterpillar ( Anticarsia gemmatalis ), the dipterans ( Culex quinquefasciatus and Aedes aegypti ) and the coleopteran ( Tenebrio molitor ). The LC 50 of the strains against second instar larvae of S. frugiperda or A. gemmatalis revealed a high potency against those insect species. The spore–crystal mixtures of the isolates were analysed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and showed similar protein pattern as the B. thuringiensis subsp. kurstaki strain HD‐1 (proteins approximately 130 and 65 kDa) for isolates S701 and S764, respectively, and only one major protein of approximately 130 kDa for isolate S1265. The polymerase chain reaction (PCR) using total DNA of the isolates and general and specific primers showed the presence of cry1Aa , cry1Ac , cry1Ia and cry2Ab genes in the two isolates serotyped as B. thuringiensis kurstaki (S701 and S764) and the presence of cry1D and cry2Ad in B. thuringiensis morrisoni S1265 strain. Scanning electron microscopy of strains S701 and S764, showed the presence of bipyramidal, cuboidal and round crystals, like in strain HD‐1 and bipyramidal and round crystals like in strain S1265.

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