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Hydrolysis of phytic acid by intrinsic plant and supplemented microbial phytase ( Aspergillus niger ) in the stomach and small intestine of minipigs fitted with re‐entrant cannulas
Author(s) -
Rapp C.,
Lantzsch H.J.,
Drochner W.
Publication year - 2001
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1046/j.1439-0396.2001.00337.x
Subject(s) - phytase , ileum , stomach , aspergillus niger , duodenum , hydrolysis , small intestine , phytic acid , zoology , jejunum , dry matter , food science , chemistry , biology , biochemistry , medicine , enzyme
Hydrolysis of phytate in the stomach and the small intestine as influenced by intrinsic plant (wheat) and supplemented microbial phytase ( Aspergillus niger ) were investigated with six minipigs (40–50 kg initial body weight) fitted with re‐entrant cannulas in the duodenum, 30 cm posterior to the pylorus (animals 1, 4, 5 and 6) and ileocecal re‐entrant cannulas, 5 cm prior the ileocecal junction (animals 1, 2 and 3), respectively. Dietary treatments were as follows: (1) diet 1, a corn‐based diet [43 U phytase/kg dry matter (DM)]; (2) diet 2, diet 1 supplemented with microbial phytase (818 U/kg DM); and (3) diet 3, a wheat‐based diet (1192 U/kg DM). At 07 30 h and 19 30 h, each animal was fed 350 g diet mixed with 1050 ml de‐ionized water. Digesta were collected continuously and completely during a 12‐h period after feeding. Mean hydrolysis rates of IP 6 in the stomach as measured at the proximal duodenum of animals 1, 4, 5 and 6 were 9.0, 77.2 and 66.2% for diet 1, 2 and 3, respectively. Microbial phytase was much more effective in phytate hydrolysis than wheat phytase. Mean IP 6 hydrolysis rates of the respective diets in the stomach and small intestine as measured at the distal ileum of animals 1, 2 and 3 were 19.0, 62.6 and 64.6% and were lower than treatment means of the stomach only. Differences existed between experimental animals with respect to their ability to hydrolyse IP 6 in the stomach independent of the presence and source of dietary phytase. Considerable amounts of hydrolysis products occurred in both the duodenal and ileal digesta when diets 2 and 3 were fed; however, only traces were determined after ingestion of diet 1. Independent of dietary treatment, four IP 5 isomers were detected, but in different amounts.