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Comparison of fatty acid composition among isolated bovine adipocytes with different sizes
Author(s) -
He M. L.,
Hidaka S.,
Matsunaga N.,
Hidari H.
Publication year - 2000
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1046/j.1439-0396.2000.00268.x
Subject(s) - adipose tissue , oleic acid , chemistry , stearic acid , composition (language) , palmitic acid , fatty acid , palmitoleic acid , adipocyte , subcutaneous fat , medicine , endocrinology , zoology , biology , biochemistry , philosophy , linguistics , organic chemistry
Summary The relationship between the diameter of adipocytes distributed in the same piece of subcutaneous adipose tissue and main fatty acid composition from fattening Holstein steers was investigated. A preliminary experiment was carried out to investigate whether the isolation process of adipocytes caused any change in the main fatty acid composition. It was shown that there was little difference in the main fatty acid composition of adipose tissue ( X ) and that of adipocytes ( Y ) isolated by this method. The regression equation was: Y = 0.97 X + 0.47, R 2 = 0.99 (p < 0.01). Adipocytes were isolated from Holstein steers at the early and late fattening stages and then separated into two fractions by using a mesh with a pore size of 50 µm. In adipocytes from Holstein steers at the late fattening stage, the large adipocytes with a mean diameter of 117.5 µm contained more (p < 0.01) myristoleic acid (14:1), palmitoleic acid (16:1) and oleic acid (18:1), and less (p < 0.01) palmitic acid (16:0) and stearic acid (18:0) than the small adipocytes with a mean diameter of 28.7 µm. Similar results have been shown in adipocytes from Holstein steers at the early fattening stages. The isolated adipocytes from finishing Holstein steers were separated into four fractions by using meshes with pore size of 25, 50, 75 and 100 µm. The mean diameter of adipocytes in fractions 1, 2, 3 and 4 were 35.2, 80.5, 113.7 and 141.7 µm, respectively. With increasing adipocyte size, the rates of myristoleic acid (14:1) and palmitoleic acid (16:1) increased (the correlation coefficients were 0.93, p < 0.01 and 0.86, p < 0.01, respectively), whereas that of palmitic acid (16:0) and stearic acid (18:0) decreased (the correlation coefficients were ‐0.44 and ‐0.80, p < 0.01, respectively). It is suggested that the size of adipocyte is one of the important determinants of fatty acid composition in subcutaneous adipocytes of Holstein steers.