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Temperature gradients between the jugular vein blood and rectum in anaesthetized, intubated pigs
Author(s) -
EinerJensen N.,
Hunter R. H. F.,
Bo gh I. B.,
Greve T.
Publication year - 1999
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1046/j.1439-0396.1999.00247.x
Subject(s) - oocyte , jugular vein , in vitro fertilisation , ovary , anatomy , human fertilization , biology , sperm , andrology , medicine , embryo , endocrinology , microbiology and biotechnology
A current focus in the Reproduction Department at the Royal Veterinary and Agricultural University (KVL) is on the relevance of pre‐ovulatory temperature gradients to procedures of in vitro oocyte maturation, fertilization, and early embryonic development. More specifically, there is a desire to establish appropriate in vitro culture temperatures for resumption of meiosis in oocyte–cumulus complexes aspirated from Graafian follicles and the subsequent oocyte–sperm suspension microdrops that are a feature of most in vitro fertilization systems. The overall objective would be to generate a high proportion of viable embryos capable of developing into a viable foetus after transplantation into a suitable recipient (foster mother). Core temperature may be defined as ‘the body’s temperature in deep structures such as the liver or heart, as opposed to the peripheral areas such as the mouth or axilla’ (C layton 1993). The core temperature is not an easy concept, as it may vary depending on the organ and type of measurement chosen. The rectal temperature is commonly used. Although it is not a ‘deep’ temperature, it is probably not very different from ‘core temperature’. Temperature gradients between different tissue compartments of the mammalian ovary have been reported several times during the last 25 years (e.g. B enoit et al. 1976; G rinsted et al. 1980; G rinsted et al. 1985). In a study on mature domestic pigs, instantaneous infrared sensing of ovarian tissue was used to reveal the surface temperature during mid‐ventral laparatomy. The ovaries were always cooler than deep rectal temperatures, and large follicles were always cooler (1.7 ± 0.4° C) than adjacent stroma (H unter et al. 1997). In an extension of these studies in domestic pigs, simultaneous measurements of jugular vein temperatures and those at a deep rectal site have also been collected. This study was conducted to investigate whether the jugular vein temperature could be taken as representative of core temperature.