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The complementary DNA sequence and polymorphisms of bovine procathepsin‐D ( CTSD )
Author(s) -
Higuchi M.,
Miyashita N.,
Nagamine Y.,
Watanabe A.,
Awata T.
Publication year - 2003
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1046/j.1439-0388.2003.00402.x
Subject(s) - biology , complementary dna , microbiology and biotechnology , brahman , single nucleotide polymorphism , genetics , nucleic acid sequence , restriction fragment length polymorphism , polymerase chain reaction , genotype , gene , breed
Summary The complementary DNA (cDNA) partial sequence of the bovine cathepsin‐D ( CTSD ) precursor, procathepsin‐D was determined. The bovine procathepsin‐D cDNA sequence and deduced protein sequence exhibited higher homology to their counterparts in sheep than other animals. A comparison of the cDNA sequence among cattle revealed nine single nucleotide polymorphisms (SNPs) in the encoding region and one nucleotide deletion in 3′‐untranslated region. Among these SNPs, a guanine (G) to adenine (A) substitution in the pro‐fragment region and a change from G to cytosine (C) in the heavy‐chain region resulted in a glycine to serine and a glycine to alanine substitution, respectively. Using polymerase chain reaction–restriction fragment length polymorphism, nine different cattle breeds were screened for the presence of both polymorphisms. It was found that three Japanese beef breeds (Japanese Black, Japanese Shorthorn and Japanese Brown) and two zebu breeds (Brahman and Santa Gertrudis) contained these polymorphisms.