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A new variant in exon VII of bovine β‐casein gene (CSN2) and its distribution among European cattle breeds
Author(s) -
JANN O.,
CERIOTTI G.,
CAROLI A.,
ERHARDT G.
Publication year - 2002
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1046/j.1439-0388.2002.00318.x
Subject(s) - exon , genetics , biology , genotyping , allele , white (mutation) , polymorphism (computer science) , single strand conformation polymorphism , allele frequency , genotype , gene , microbiology and biotechnology
An undescribed bovine CSN2 variant was detected and characterized. It is based on a mutation in exon VII, which leads to a substitution of the aminoacid 93 (Met→Leu) in comparison to allele CSN * A 2 . The new allele was named CSN2 * I . A PCR‐SSCP based DNA‐test for the differentiation of the common alleles A 1 , A 2 , A 3 , B, C, and of the new variant I was developed. Genotyping of 318 unrelated animals belonging to seven European breeds (Italian Holstein Friesian, Italian Red Pied, Piemontese, Angler, Maremmana, Polish Red and White, Polish Red) showed a wide distribution of the new variant with a frequency up to 0.14 in Italian Red Pied.