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Identification of the variant C of β‐lactoglobulin in sheep using a polymerase chain reaction‐restriction fragment length polymorphism method
Author(s) -
Anton By I.,
Zsolnai A.,
F L.,
ésüs .
Publication year - 1999
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1046/j.1439-0388.1999.00207.x
Subject(s) - genotype , genotyping , biology , restriction fragment length polymorphism , polymerase chain reaction , polymorphism (computer science) , microbiology and biotechnology , beta lactoglobulin , allele , genetics , polymerase , dna , whey protein , biochemistry , gene
β‐Lactoglobulin (β‐LG) is the major whey protein in the milk of ruminants and is able to bind and transport small hydrophobic molecules. However, its biological role is mainly unknown (G odovac ‐Z immermann 1988). Previously three genetic variants have been found in sheep: A, B and C. The genetic variants A and B differ at amino acid position 20, where variant A has a His and variant B has a Thr (K olde and B raunitzer 1983). The variant C is a subtype of variant A with a single amino acid exchange of Arg→Glu at position 148 (E rhardt et al. 1989). The genotype β‐LG BB was found to be associated with higher milk yield, whereas genotypes AA and AB had a higher milk protein and casein content as well as yielding more curd (G arzon and M artinez 1992). No data is available concerning the relationship between the β‐LG C allele and production traits or milk properties. Since DNA‐based genotyping has already been performed for alleles A and B (S chlee et al. 1993), the aim of the present study was to develop a DNA‐based method for identifying the β‐LG C variant. However polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) typing has been established (P rinzenberg and E rhardt 1999) recently, and this study shows an alternative method to detect β‐LG C.