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Polymerase chain reaction primers for the detection of Ceratobasidium bicorne (uninucleate Rhizoctonia )
Author(s) -
HANTULA J.,
LILJA A.,
VEIJALAINEN A.M.
Publication year - 2002
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1046/j.1439-0329.2002.00292.x
Subject(s) - biology , scots pine , rhizoctonia , picea abies , botany , inoculation , pinus <genus> , mycelium , horticulture , rhizoctonia solani
Summary An uninucleate Rhizoctonia sp. causes a root dieback on Norway spruce ( Picea abies ) and Scots pine ( Pinus sylvestris ) seedlings in forest nurseries. Primers specific to this pathogen were designed, and they yielded an amplification product from mycelial cultures only when uninucleate Rhizoctonia sp. was used as a template. Inoculated Norway spruce and Scots pine seedlings with visible symptoms gave a clear and reproducible band. The same band appeared also from Norway spruce seedlings growing in inoculated peat or compost but lacking conspicuous symptoms. One of the benefits of the DNA‐based test was its higher sensitivity compared with the traditional isolation. The results are also available more quickly.