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Methods for isolating the alder Phytophthora
Author(s) -
STREITO J.C.,
JARNOUEN DE VILLARTAY G.,
TABARY F.
Publication year - 2002
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1046/j.1439-0329.2002.00283.x
Subject(s) - alder , biology , bark (sound) , corn meal , phytophthora , fungus , agar , botany , isolation (microbiology) , pathogen , alnus glutinosa , cutting , horticulture , microbiology and biotechnology , ecology , food science , bacteria , genetics
Phytophthora disease of alder ( Alnus spp.) is now widespread in Europe. In France, difficulties were initially encountered in attempts to isolate the pathogen from necrotic bark tissue but, with experience, a high degree of success was achieved by direct plating onto agar media. Both corn meal agar and a selective medium derived from PARBHy were satisfactory. Success depended largely on the activity of the fungus in the bark tissue and some evidence was obtained that this varied from year to year. However, autumn was found to be a generally good period for isolation. It is possible to store collected samples at 7 ± 4°C for some days. The alder Phytophthora was isolated from the water of two rivers with a baiting method using alder twigs but the efficiency of the method was low.