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Unterscheidung der Intersterilitätsgruppen bei Heterobasidion annosum mit Hilfe von Polymorphismen der ribosomalen IGS1‐Region
Author(s) -
Kasuga T.,
Mitchelson K. R.
Publication year - 2000
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1046/j.1439-0329.2000.00216.x
Subject(s) - heterobasidion annosum , biology , restriction fragment length polymorphism , ribosomal rna , genetics , ribosomal dna , population , microbiology and biotechnology , gene , polymerase chain reaction , botany , picea abies , phylogenetics , demography , sociology
Summary The intergenic spacer region 1 (IGS1) of the nuclear ribosomal RNA gene repeat was examined in three European intersterility groups of the wood‐rot fungus Heterobasidion annosum . The IGS1 elements were informative for restriction fragment length polymorphism (RFLP) fingerprinting the fungal intersterility groups following digestion with two different four‐cutter restriction endonucleases. The fingerprints of isolates from the same intersterility group from different localities shared some fragments in common, but also showed specific fragments suggesting that RFLP of the IGS1 region might be used in population studies. RFLP patterns between isolates from different intersterility groups differed more markedly. RFLP were also detected in the IGS1 region of North American strains of H. annosum and in the related species Heterobasidion araucariae . The results indicate that ribosomal IGS1 region fingerprinting could be a useful tool to identify the fungal intersterility group in Heterobasidion annosum .