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Use of random amplified polymorphic DNA (RAPD) for detection of genetic variation and proof of the heterothallic mating system in Hypoxylon mediterraneum
Author(s) -
Vannini A.,
Mazzaglia A.,
Anselmi N.
Publication year - 1999
Publication title -
european journal of forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 0300-1237
DOI - 10.1046/j.1439-0329.1999.00145.x
Subject(s) - heterothallic , rapd , biology , mating type , genetic variation , genetic variability , fungus , genetics , genetic diversity , evolutionary biology , botany , population , gene , genotype , demography , sociology
Summary The efficiency of RAPD analysis in the evaluation of genetic variability in Hypoxylon mediterraneum has been tested on 49 monoascospore isolates of the fungus from different localities. A large genetic variability was detected within single stromata and among monoascospore isolates in a restricted area. The large variability in restricted populations can be explained by the heterothallic mating system and the life cycle of the fungus and its ecological adaptations. RAPD markers can discriminate monoascospore isolates both in the single stromata and among stromata. The application of RAPD markers for epidemiological studies is discussed.