Premium
Partial cDNA sequence analysis of the boar proacrosin‐binding protein
Author(s) -
Huh K.,
Yi L. S. H.
Publication year - 2001
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1046/j.1439-0272.2001.00466.x
Subject(s) - boar , complementary dna , oligonucleotide , microbiology and biotechnology , biology , agarose gel electrophoresis , peptide sequence , dna , puc19 , sequence analysis , chemistry , biochemistry , gene , genetics , plasmid , semen
Summary. A partial cDNA sequence of the boar 28, 29‐kDa proacrosin‐binding protein was determined. The desired sequence was amplified and obtained by RT‐PCR followed by nested PCR. The oligonucleotide primers for RT‐PCR were designed by deduction from the internal amino acid sequence of the boar proacrosin‐binding protein. An approximately 1‐kbp DNA fragment was analysed by agarose gel electrophoresis, in which the boar testicular total RNA or poly(A) + RNA was used as a template for RT‐PCR followed by nested PCR. This PCR product was inserted into a TA vector. The DNA sequence of the inserted PCR product was then determined by the chain termination method.