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Enrichment of high‐quality spermatozoa in bovine semen: relative effectiveness of three filtration matrixes
Author(s) -
Cisale H. O.,
Fischman M. L.,
Blasi C. D.,
Fernandez H. A.,
Gledhill B. L.
Publication year - 2001
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1046/j.1439-0272.2001.00413.x
Subject(s) - filtration (mathematics) , semen , chromatography , semen quality , size exclusion chromatography , chemistry , artificial insemination , sephadex , centrifugation , andrology , biology , mathematics , medicine , biochemistry , statistics , pregnancy , genetics , enzyme
Summary. To be practical, any method for improving bull semen must yield a large quantity of motile spermatozoa. Some separation methods based on physical properties, e.g. filtration, chromatography, centrifugation, washing and pooling, have been reported as satisfactory, but generally are not repeatable. Nevertheless, filtration methods appear to allow the attainment of an acceptable number of spermatozoa, thus allowing such a technique to be introduced in the production of standard bovine semen doses for artificial insemination. The aim of this work was to evaluate systematically the relative effects of three filtration matrixes (silica oxide, glass beads or Sephadexä ™ ) on the improvement of whole ejaculate quality. Analysis of the type of matrix and the volume and height of the filtration column was performed. The only characteristic of the columns that appears to influence ejaculate quality after filtering is the matrix volume. While all matrixes produced improvement of semen quality, Sephadex TM was better than the other matrixes tested. An explanation for the mechanism of column filtration is proposed.