Premium
A new method to produce equally sized hemizonae pellucidae for the hemizona assay
Author(s) -
Montag M.,
Lemola R.,
Van Der Ven H.
Publication year - 2000
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1046/j.1439-0272.2000.00334.x
Subject(s) - zona pellucida , zona , laser , oocyte , materials science , biomedical engineering , biology , microbiology and biotechnology , optics , medicine , physics , immunology , embryo , virus , viral disease
The hemizona assay (HZA) is a valuable tool to study the binding potential and interaction of spermatozoa with the zona pellucida. Its accuracy strongly depends on the use of equally sized hemizonae. Usually, manipulator‐guided microblades are used for cutting the zona pellucida. Recently, lasers were introduced for precise local thermolysis of the zona. The use of a 1.48 μm diode laser for the generation of hemizonae from human oocytes was investigated. This laser allowed drilling of equally sized hemizonae which were used for hemizona binding assays. It is concluded that the 1.48 μm diode laser system can be applied for the production of hemizonae. The method is easy to perform and offers a fast and efficient access to hemizonae of identical size.