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Galanin‐Immunoreactive Cells and Their Relation to Calcitonin Gene‐Related Peptide‐, Substance P‐ and Somatostatin‐Immunoreactive Cells in Rat Lumbar Dorsal Root Ganglia
Author(s) -
Yoon Y. S.,
Hwang I. K.,
Lee I. S.,
Suh J.G.,
Shin J. W.,
Kang T.C.,
Oh Y. S.,
Won M. H.
Publication year - 2003
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1046/j.1439-0264.2003.00425.x
Subject(s) - calcitonin gene related peptide , galanin , neuropeptide , substance p , calcitonin , somatostatin , medicine , endocrinology , immunocytochemistry , neuropeptide y receptor , biology , chemistry , receptor
Summary We report upon the distribution of galanin‐immunoreactive (GAL‐IR) cells in the lumbar dorsal root ganglia (DRG) of the rat, and upon the distribution of GAL‐IR cells, which also contain calcitonin gene‐related peptide (CGRP)‐, substance P (SP)‐ and somatostatin (SOM)‐immunoreactivity. Neuropeptide‐immunoreactive lumbar DRG cells were 55.8% for CGRP, 12.7% for SP, and 6.5% for GAL in lumbar DRG cells. There was no significant difference between the right and left DRGs (L 1 −L 6 ) for any neuropeptide‐immunoreactive cell ( P <0.01). In terms of size distribution, CGRP‐immunoreactive cells were identified below 1500 μ m 2 , and SP‐, and GAL‐IR cells below 600 μm 2 . Neuropeptide immunoreactive cells showed various immunoreactivities in the cytoplasm according to each neuropeptide. CGRP and SP immunoreactive cells were colocalized with GAL immunoreactive cells in the serial sections about 83.3 and 60% respectively, but SOM colocalizing with GAL‐IR cells were not in evidence. The current results confirm and extend previous results, and show that neuropeptides can coexist in single sensory neurones of the rat DRG. In addition, our results demonstrate that the normal distribution of some neurotransmitters modulating sensory action in Wistar Kyoto rat, make this model more prone to develop neuropathic pain than Sprague‐Dawley rat.