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Ultrastructural Changes in Calvarial Osteoblast Cytoskeleton after Prostaglandin E 2 Administration in Rats
Author(s) -
Yamakura K.,
Watanabe H.,
Abe S.,
Yanagisawa T.
Publication year - 2001
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1046/j.1439-0264.2001.00340.x
Subject(s) - microfilament , cytoskeleton , ultrastructure , microbiology and biotechnology , cytoplasm , osteoblast , osteoid , chemistry , cell , biology , anatomy , in vitro , biochemistry
Recent studies on the cytoskeleton of osteoblasts have been made mainly using cultured cells. However, the morphology of cultured cells may be altered during subculture. Therefore, cytoskeletal changes of calvarial osteoblasts were investigated in situ by electron microscopy using the detergent perfusion method to preserve cell morphology as well as selectively observing the cytoskeleton in the presence of a high concentration of prostaglandin E 2 (PGE 2 ) in the calvarial periphery in rats. Rats were perfused with a mixture of Triton X‐100 and glutaraldehyde, then the cytoskeleton was observed by transmission electron microscopy. In osteoblasts of the control group, thick bundles of microfilaments ran parallel to the long axis of the cells immediately below the cell membrane adjacent to the osteoid layer. In contrast, in the osteoblasts of the PGE 2 ‐administered group, the external morphology was changed to an asteroid or cubic shape, and thick bundles of microfilaments immediately below the cell membrane adjacent to the osteoid were not observed, although microfilament fibres, with a diameter of 5–6 nm, were observed in the cytoplasm.