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Comparison of Localization of the Neurokinin 1 Receptor with AMPA‐type Glutamate Receptors in the Rat Spinal Cord
Author(s) -
Nazli M.,
Morris R.
Publication year - 2000
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1046/j.1439-0264.2000.00270.x
Subject(s) - ampa receptor , spinal cord , receptor , glutamate receptor , gdf7 , neuroscience , biology , protein subunit , lumbar spinal cord , microbiology and biotechnology , biochemistry , gene , embryonic stem cell
Summary With the cloning of the α‐amino‐3‐hydroxy‐5‐methyl‐4‐isaxole propionic acid (AMPA)‐type receptor subunits, it is now possible to localize these receptor subunits in the spinal cord. Comparison of the neurokinin 1 receptor distribution with that of non‐ N ‐methyl‐ d ‐aspartate glutamate receptor subunits (GluR1–4), considered to be AMPA‐type, was investigated in rat spinal cord by immunocytochemical methods. Different patterns of immunolabelling were observed with the antibodies to the GluR1, GluR2/3 and GluR4 subunits in the lumbar spinal cord. Immunolabelling with antibodies to both GluR1 and GluR2/3 revealed intensive staining in the dorsal horn, while staining for GluR2/3 and GluR4 was dense in the motor neurons of the ventral horn. These results suggest that in the rat spinal cord AMPA‐type receptors vary their composition according to the region where they are expressed. Neurokinin‐1‐receptor‐expressing neurons in the dorsal horn do not appear to express the GluR4 subunit, however, whether they express the GluR1, GluR2/3 receptors subunits could not be determined.