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Immunohistochemistry of carbonic anhydrase isozymes (CA‐I, II and III) in canine salivary glands: a distributional and comparative assessment
Author(s) -
Asari M.,
Kimura H.,
Ichihara N.,
Kasuya T.,
Nishita T.
Publication year - 2000
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1046/j.1439-0264.2000.00234.x
Subject(s) - serous fluid , isozyme , ductal cells , immunostaining , carbonic anhydrase , salivary gland , antiserum , submandibular gland , saliva , duct (anatomy) , biology , myoepithelial cell , immunohistochemistry , chemistry , pathology , microbiology and biotechnology , endocrinology , anatomy , antigen , enzyme , biochemistry , medicine , immunology
The immunohistolocalization of carbonic anhydrase isozymes (CA‐I, II, III) in canine salivary glands was studied using antiserum against CA‐I, II, III. In parotid glands, immunostaining intensely localized cytosolic CA‐II antiserum throughout the cytoplasm of acinar secretory cells and ductal epithelial cells, especially in the striated duct region. CA‐III reactivity in the glands was only seen selectively at the intercalated ductal cells. In contrast, no immunoreaction localized CA‐I in the gland. In the submandibular and sublingual glands, CA‐I, II, and III were all observed in the ductal segments of the glands, whereas serous demilune appeared devoid of all three cytosolic CA isozymes. In contrast, in zygomatic glands (i.e. dorsal buccal glands) all CA isozymes were observed in both serous demilune and ductal segments. In all of the salivary glands examined, no mucous acinar cells were found to be reactive for any CA.