Open AccessA new structural type for Haemophilus influenzae lipopolysaccharide
Author(s) -
Månsson Martin,
Bauer Sebastian H. J.,
Hood Derek W.,
Richards James C.,
Moxon E. Richard,
Schweda Elke K. H.
Publication year - 2001
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.2001.02094.x
Subject(s) - heptose , haemophilus influenzae , lipid a , lipopolysaccharide , mutant , chemistry , stereochemistry , microbiology and biotechnology , biochemistry , gene , biology , antibiotics , endocrinology
Structural elucidation of the sialylated lipopolysaccharide (LPS) of non‐typeable Haemophilus influenzae (NTHi) strain 486 has been achieved by the application of high‐field NMR techniques and ESI‐MS along with composition and linkage analyses on O‐deacylated LPS and oligosaccharide samples. It was found that the LPS contains the common element of H. influenzae , l ‐α‐ d ‐Hep p ‐(1→2)‐[ P Etn→6]‐ l ‐α‐ d ‐Hep p ‐(1→3)‐[β‐ d ‐Glc p ‐(1→4)]‐ l ‐α‐ d ‐Hep p ‐(1→5)‐[ PP Etn→4]‐α‐Kdo p ‐(2→6)‐Lipid A, but instead of glycosyl substitution of the terminal heptose residue (HepIII) at the O2 position observed in other H. influenzae strains, HepIII is chain elongated at the O3 position by either lactose or sialyllactose (i.e. α‐Neu5Ac‐(2→3)‐β‐ d ‐Gal p ‐(1→4)‐β‐ d ‐Glc p ). The LPS is substituted by an O‐acetyl group linked to the O2 position of HepIII and phosphocholine ( P Cho) which was located at the O6 position of a terminal α‐ d ‐Glc p residue attached to the central heptose, a molecular environment different from what has been reported earlier for P Cho. In addition, minor substitution by O‐linked glycine to the LPS was observed. By investigation of LPS from a lpsA mutant of NTHi strain 486, it was demonstrated that the lpsA gene product also is responsible for chain extension from HepIII in this strain. The involvement of lic1 in expression of P Cho was established by investigation of a lic1 mutant of NTHi strain 486.