Open AccessEpisomal vectors for gene expression in mammalian cells
Author(s) -
Van Craenenbroeck Kathleen,
Vanhoenacker Peter,
Haegeman Guy
Publication year - 2000
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.2000.01645.x
Subject(s) - biology , plasmid , gene , gene expression , virus , vector (molecular biology) , heterologous expression , heterologous , transfection , dna , genetic enhancement , computational biology , genetics , virology , microbiology and biotechnology , recombinant dna
An important reason for preferring mammalian cells for heterologous gene expression is their ability to make authentic proteins containing post‐translational modifications similar to those of the native protein. The development of expression systems for mammalian cells has been ongoing for several years, resulting in a wide variety of effective expression vectors. The aim of this review is to highlight episomal expression vectors. Such episomal plasmids are usually based on sequences from DNA viruses, such as BK virus, bovine papilloma virus 1 and Epstein–Barr virus. In this review we will mainly focus on the improvements made towards the usefulness of these systems for gene expression studies and gene therapy.