Open AccessExpression patterns of the regulatory proteins G protein‐coupled receptor kinase 2 and β‐arrestin 1 during rat postnatal brain development
Author(s) -
Penela Petronila,
ÁlvarezDolado Manuel,
Muñoz Alberto,
Mayor Federico
Publication year - 2000
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.2000.01484.x
Subject(s) - g protein coupled receptor kinase , beta adrenergic receptor kinase , g protein coupled receptor , arrestin , receptor , biology , medicine , endocrinology , microbiology and biotechnology , g protein , signal transduction , neuroscience , genetics
G protein‐coupled receptor kinase 2 (GRK2) and β‐arrestin 1 are key regulatory proteins that modulate the desensitization and resensitization of a wide variety of G protein‐coupled receptors (GPCRs) involved in brain functions. In this report, we describe the postnatal developmental profile of the mRNA and protein levels of GRK2 and β‐arrestin 1 in rat brain. The expression levels of GRK2 and β‐arrestin 1 display a marked increase at the second and third week after birth, respectively, consistent with an involvement of these proteins in brain maturation processes. However, the expression attained at birth and during the first postnatal week with respect to adult values (45–70% for GRK2, ≈ 30% for β‐arrestin 1) is relatively high compared to that reported for several GPCRs, indicating the existence of changes in the ratio of receptors to their regulatory proteins during brain development. On the other hand, we report that experimental hypothyroidism results in changes in the patterns of expression of GRK2 and β‐arrestin 1 in cerebral cortex, leading to a 25–30% reduction in GRK2 levels at several stages of development. Such changes could help to explain the alterations in GPCR signaling that occur during this pathophysiological condition.